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BioGems重組人轉(zhuǎn)鐵蛋白的性質(zhì)

更新時間:2018-04-18瀏覽:1949次

BioGems重組人轉(zhuǎn)鐵蛋白是在無動物源的GMP車間生產(chǎn)的,活性相當于人血清鐵飽和的轉(zhuǎn)鐵蛋白(holo transferrin),純度≥ 95% 適用于細胞培養(yǎng)。

 

BioGems重組人轉(zhuǎn)鐵蛋白參數(shù)

別名 Serotransferrin(血清轉(zhuǎn)鐵蛋白), TF(轉(zhuǎn)鐵蛋白), TFQTL1, PRO2086, PRO1557

 

表達系統(tǒng) Oryza Sativa 水稻

 

氨基酸序列 VPDKTVRWC AVSEHEATK CQSFRDHMK SVIPSDGPS VACVKKASY LDCIRAIAA NEADAVTLD AGLVYDAYL APNNLKPVV AEFYGSKED PQTFYYAVA VVKKDSGFQ MNQLRGKKS CHTGLGRSA GWNIPIGLL YCDLPEPRK PLEKAVANF FSGSCAPCA DGTDFPQLC QLCPGCGCS TLNQYFGYS GAFKCLKDG AGDVAFVKH STIFENLAN KADRDQYEL LCLDNTRKP VDEYKDCHL AQVPSHTVV ARSMGGKED LIWELLNQA QEHFGKDKS KEFQLFSSP HGKDLLFKD SAHGFLKVP PRMDAKMYL GYEYVTAIR NLREGTCPE APTDECKPV KWCALSHHE RLKCDEWSV NSVGKIECV SAETTEDCI AKIMNGEAD AMSLDGGFV YIAGKCGLV PVLAENYNK SDNCEDTPE AGYFAIAVV KKSASDLTW DNLKGKKSC HTAVGRTAG WNIPMGLLY NKINHCRFD EFFSEGCAP GSKKDSSLC KLCMGSGLN LCEPNNKEG YYGYTGAFR CLVEKGDVA FVKHQTVPQ NTGGKNPDP WAKNLNEKD YELLCLDGT RKPVEEYAN CHLARAPNH AVVTRKDKE ACVHKILRQHLFGSNV TDCSGNFCL FRSETKDLL FRDDTVCLA KLHDRNTYE KYLGEEYVK AVGNLRKCS TSSLLEACT FRRP

 

質(zhì)檢 通過N端和質(zhì)譜分析進行驗證(適用時)。Verified by N-terminal and Mass Spectrometry analyses(when applicable).

 

純度 ≥ 95% by SDS-PAGE.

 

內(nèi)毒素水平 Endotoxin level is < 1 EU/mg.

 

生物活性 在無血清添加培養(yǎng)基中通過與天然人轉(zhuǎn)鐵蛋白在雜交瘤細胞的抗體生產(chǎn)中比較來證實生物學活性。Biological activity verified by comparison against native human transferrin in the antibody production of hybridoma cells in serum-free supplemented media.

 

處理 0.2μm滅菌過濾。從磷酸鈉和氯化鈉溶液(pH7.0)凍干。